PhysioMimix® OOC Microphysiological Systems
Transforming the way human-relevant preclinical data is generated
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Generate human-specific pre-clinical safety and efficacy data with pioneering PhysioMimix single- and multi-organ-on-a-chip solutions.
PhysioMimix organ-on-a-chip (OOC) instruments allow researchers to culture microtissues that mimic the structure and function of human tissues and organs.
A wide range of routine laboratory assays can be run to identify drug targets, understand drug interactions and mechanisms of toxicity to more closely predict clinical outcomes.
PhysioMimix is a suite of hardware, consumables and assay protocols that enable the co-culturing of human cells straight out of the box. The perfused system provides accurate fluidic flow that replicates the bloodstream promoting 3D organ model formation and supports the inclusion of circulating immune cells to mimic the immune system.
By improving the in vivo predictivity of in vitro data, PhysioMimix OOC systems help companies like yours to develop safe and efficacious therapeutics, faster and more cost-effectively, while reducing your dependence on animal usage.
How translatable is your data?
Human-relevant data generated by the PhysioMimix OOC complements that derived using entrenched 2D cell culture and animal studies to support more confident decision-making across many drug discovery stages from target discovery right through to preclinical development.
Limitations of current techniques
- In vitro 2D cell culture models lack physiological relevance
- In vivo animal models lack human relevance; studies are slow, expensive, and ethically undesirable
- Inter-species differences limit the suitability of animals for testing human-specific drug modalities
Advancements with PhysioMimix
- Faithfully recapitulate the phenotype and function of human organs and tissues in vitro
- Addresses the relevance limitations of standard preclinical models to validate or query their results
- Provides a rapid, usable, cost-effective and more human-relevant alternative
What’s required to accurately model and measure human physiology?
PhysioMimix OOC enables:
- the co-culturing of human cells to faithfully recreate human organs and tissues
- perfused scaffolds to promote 3D organ model formation
- compatibility with inserts for apical and basolateral tissue growth to recreate a biological barrier
- recirculating fluidic flow delivering biomechanical stimulus, oxygen, and nutrients
- adjustable inter- and intra-organ flow rates to enhance physiological relevance
- viability, function, and phenotype to be maintained over many weeks to enable longer-term chronic disease and dosing studies
- deep mechanistic insights via large sampling volume (up to 1 mL) and individual microtissue analysis
- modeling of the immune system via the inclusion of circulating and tissue-specific immune cells
Preclinical toolbox comparison
How does OOC compare to conventional preclinical approaches?
| In vitro 2D cell culture | In vitro 3D spheroid | In vivo animal models | Organ-on-a-chip | |
| Human relevance | ||||
| Complex 3D organs and tissues | ||||
| (Blood)/Flow perfusion | ||||
| Innate & adaptive immune system | ||||
| Longevity | < 7 days | < 7 days | > 4 weeks | ~ 4 weeks |
| Acute and chronic dosing | ||||
| New drug modality compatibility | LOW | MEDIUM | LOW | MEDIUM / HIGH |
| Throughput | ||||
| Time to result | FAST | FAST | SLOW | FAST |
| High content data |
Which of our microphysiological systems meets your needs?
PhysioMimix Single-organ Standard System
Entry-level system that enables the creation of in vitro 3D single-organ models to provide deep insights into drug, or disease mechanism of action.
Enables insightful decision making by delivering clinically translatable human data.
Generate Liver-on-a-chip models that mimic the human liver microarchitecture using the PhysioMimix Liver-12 plate.
Generate barrier models (such as Gut- or Lung-on-a-chip) on Transwell® inserts using the PhysioMimix Barrier plate.
PhysioMimix Single-organ HT System
Next-generation system that additionally provides:
6x higher throughput * for comparative studies evaluating drug safety, efficacy and potency.
8x reduction in cost/sample* to reduce adoption barriers.
Enhanced data robustness and reproducibility capabilities for data confidence.
Delivers clinically translatable human data earlier in discovery than ever before.
Compatible with the NEW PhysioMimix Liver-48 plate, plus those of the Single-organ Standard System.
*versus a single-chip alternative
PhysioMimix Multi-organ Standard System
Providing additional functionality over the Single-organ Standard System, the multi-organ variant enables you to interconnect our liver model with an additional organ, such as the gut or lung,
By mimicking how organs interact and communicate as part of a complex system, you benefit from a deeper human-specific mechanistic understanding of disease states and drug behavior.
Comptible with the PhysioMimix Dual-organ plate, plus those of the Single-organ Standard System
PhysioMimix OOC range by task
| Single-organ Systems | Multi-organ System | ||
| Standard | HT | Standard | |
| Mechanistic insights | |||
| Safety, efficacy & potency | |||
| Inter-organ crosstalk | |||
Bringing the benefits of Organ-on-a-chip to more of drug discovery
Customer feedback
The CN Bio MPS system allows hepatic cultures to be grown for weeks with low signs of cytotoxicity or hepatocytes de-differentiation detectable; CN Bio has developed an efficient proprietary protocol to model NASH that shows really high homology with murine models of NASH in terms of transcriptome, inflammatory profiling and pathophysiological events crucial for disease progression
The hepatic co-cultures of hepatocytes, Kupffer and stellate cells that I have tested also behave as expected in vivo with regards to intracellular signalling pathways (such as TGFβ signalling) thus allowing the modeling of chronic liver disease pathophysiology.
I think that the PhysioMimix OOC system will become a “must-have” piece of equipment for labs focussing on liver pathophysiology and tissue-to-tissue interactions.
Michele Vacca, MD PhD
Clinician Research Associate at the Institute of Metabolic Science, University of Cambridge
View more PhysioMimix OOC reviews on SelectScience
Application areas
Disease modeling
Our models functionally mimic the organ and give a realistic expression of disease phenotypes for improved efficacy assessments
Safety toxicology
By closely mimicking in vivo function, our models better predict drug safety to support and accelerate drug development.
ADME
Single- and multi-organ models closely predict human in vivo pharmacokinetics for informed insights into the body’s effect on drugs.
Why choose PhysioMimix?
Lab benchtop ready
Compact and compatible with existing equipment
User friendly
Program and start a run in less than 1 minute
Open-well design
Supports your 2D to 3D cell culture transition
Real-time monitoring
Remove samples for analysis, experiments continue to run
“Set-and-Run” Perfusion
Long-term automated experiments with minimal user input
Tissues and cells
Compatible with a range of pre-formed tissues and cell types for ultimate flexibility
Multi-organ compatible
Connect two organs via microfluidics to study crosstalk
Reduced cost/chip
More experiments, same cost reduces adoption barriers
Data confidence
In-plate replicates, controls and dose-responses
Higher throughput
Generate OOC insights earlier than ever before
