Generate more physiologically relevant barrier models in vitro with the PhysioMimix Barrier plate
PhysioMimix Barrier plates enable primary human cells to be cocultured on the apical and basolateral sides of a Transwell® insert to recreate biological barrier models.
Epithelial and endothelial cells may be cultured at an air-liquid interface (ALI) or in the presence of media. Circular flow perfusion on the basolateral side recreates a physiologically relevant microenvironment within each of the plate’s 12 chips.
This provides continuous access to oxygen and nutrients for long-term culture and facilitates the creation of 3D-like biological barrier tissue with more in vivo representative morphology and integrity than static cultures.
These plates accommodate standard 0.33 cm2 Transwell inserts and are
- primary cells
- induced-pluripotent stem cells
- immortalized cell lines
- circulating immune cells
- commercial inserts (such as MucilAir® or EpiSkin®).
Barrier plates are designed for use with PhysioMimix OOC systems and can be used for a variety of applications.