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Drug-induced liver injury (DILI)

Can DILI be detected early with increased sensitivity?


Toxicity in the liver remains a major cause of drug attrition, with existing in vitro and in vivo models failing to capture DILI responses.

Most medications are metabolized in the liver, which makes it one of the primary tissues affected by adverse drug reactions. Earlier identification means that promising drugs can undergo structural modifications to address toxicity issues to lower attrition rates. To do so requires preclinical assays with greater human relevance and sensitivity.

Our solution

Our PhysioMimix™ DILI Assay allows you to predict human liver responses to acute and chronic drug exposure, in the presence/absence of underlying disease.

This in vitro assay consists of human primary liver parenchymal and non-parenchymal cells (NPCs) cultured in 3D under perfusion to form tissues from which it is possible to detect functional liver-specific endpoints (including clinical biomarkers such as albumin and ALT/AST). The assay enables the sensitive assessment of compound toxicity with full dose–response curves and, by incorporating Kupffer cells, allows for the evaluation of immune-mediated toxicity.

Both acute and chronic exposure to compounds can be assessed by comparing responses to single- and multi-dosed tissues for up to four weeks. The assay produces highly functional and metabolically active liver tissues for DILI screens to give deep mechanistic insights into the toxicity profile of a compound.

DILI cells

Studying DILI

Limitations with current techniques

  • Poor in vitro to in vivo translation
  • Fail to provide deep mechanistic insights into the cause of toxicity
  • Many advanced in vitro models are low throughput limiting the number of data points
  • Challenging to run chronic exposure assays
  • Production of toxic phase I and II metabolites is not possible

Advancements with PhysioMimix OOC

  • Translate data from the lab to the clinic by using clinical biomarker reporting
  • Assess multiple cellular endpoints to determine the mechanism of toxicity
  • Generate full dose–response curves for multiple compounds per plate
  • Long-term liver cultures facilitate prolonged repeat dosing for weeks
  • Correlate the production of toxic phase I and II metabolites with cell health measurements

Generate high content data for a complete DILI profile


Our sensitive DILI assay provides high-content data from every replicate allowing the exploration of mechanisms of DILI alongside the assessment of a range of cell health markers.

Rowe et al., 2018

DILI profile pie chart
DILI assay graph

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Improve in vitro to in vivo translation


Improved data translatability from the lab to the human is achieved through full dose–response experiments measuring clinical markers, such as ALT/AST, and has been tested using a range of toxic therapeutics.

Improved in vitro to in vivo translation line grpahs
Data graph

Access our DILI Service


Get instant access to the PhysioMimix DILI Assay via our CRO Service. Through a collaborative approach, our experts work with you to plan and execute your study.

Standard and bespoke projects are carried out by our dedicated team of scientists in our CRO facility providing you with actionable data within weeks.

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Add PhysioMimix OOC into your lab


Harness the power of PhysioMimix OOC in your own lab with the purchase of a single- or multi-organ microphysiological system.

With a growing community of users and support from our experts, there has never been a better time to transition into 3D cell culture.

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Featured resources

Application notes

New AppNOTE |

Human liver microphysiological system for predicting the drug-induced liver toxicity of differing drug modalities

Application notes

MPS Fatty Liver graphic |

Microphysiological system for studying fatty liver disease and its impact on drug-induced liver injury

Blogs

blog lung in crisis |

De-risking drug-induced liver injury through the predictive power of organ-on-a-chip

View all Drug-induced liver injury Service related resources

Speak to our experts

Speak directly with one of our OOC experts to see how our products and services can support your studies

Request a meeting

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Latest news

  • The FDA further expands collaboration with CN Bio to evaluate the PhysioMimix Multi-organ microphysiological system January 17, 2023
  • The U.S. FDA Modernization Act 2.0. Now the animal testing mandate is removed, learn what can be embraced in its place. January 9, 2023
  • CN Bio appoints Dr Paul Brooks as Chief Executive Officer December 19, 2022

Upcoming events

MPS World Summit 2023 June 26-30, 2023

SLAS Europe 2023 May 22-26, 2023

SLAS2023 February 25 - March 1, 2023