Liver-on-a-chip models
In vitro liver models with unrivalled human relevance
Corporate Video
Our predictive human Liver-on-a-chip (or Liver chip), models are cultured in Multi-chip Liver plates by the PhysioMimix® OOC range of microphysiological systems.
They recreate the multi-cellular architecture of the liver, enabling drug metabolism and transport, drug-induced liver injury and chronic liver disease studies.
Getting started is easy. We’ve made the complex simple by supplying fully validated SOPs, 3D validated cells and “in-a-box” complete solution kits.
Why use our Liver-on-a-chip models?
Multi-cell co-cultures
Combine hepatic and immune cell types to recreate the liver microenvironment
3D microtissues
Hepatocytes adhere to create highly polarised functional microtissues supported by scaffolds
Fluidic flow
Provides essential nutrients, O2 and biomechanical stimuli to promote culture longevity
Physiological cues
Accurately model a variety of diseases such as NAFLD/NASH, Hepatitis B and liver cancer
High metabolic activity
Permits human phase I and II metabolism, drug transport, and metabolite-induced toxicity studies
Flexible cell compatibility
Primary cells, immortalised cell lines, stem-cell or patient-derived tissue slices
Measurement of clinical biomarkers
Enables a smooth transition of data between the laboratory and the clinic
High inter- and intra-plate reproducibility
High inter- and intra-plate reproducibility delivers robust and reliable data
Dynamic 3D microenvironment
Primary hepatocytes and non-parenchymal cells are cultured to form 3D microtissues in bespoke engineered scaffolds. Microtissues are continuously perfused by media to mimic blood flow.
The dynamic 3D microenvironment of the Liver-on-a-chip promotes the viability and functionality of liver cells, enabling their long-term culture for up to four weeks.
Clinical translatability
Using Liver-on-a-chip models, multiple clinically relevant biomarkers can be monitored over time from single samples. The model expresses human-relevant levels of phase I/II enzymes and transporters.
They permit an almost limitless number of end points to be profiled from acute and chronic studies and improve the predictive performance of in vitro assays by delivering data proven to translate into clinical outcomes.
Ultimate flexibility
Work the way you want to with the PhysioMimix suite of hardware, protocols and consumables.
- Application-based SOPs and primary human 3D validated cells support the easy re-creation of our industry validated mono- and duo-culture models.
- Our NASH-in-a-box kit provides an off-the-shelf solution to fast-track the adoption of our human Non-alcoholic steatohepatitis model in your laboratory
- Adapt the model to match your research needs. Incorporate physiological combinations of additional cell types such as non-parenchymal cells (NPCs), immune cells (innate/adaptive), or exogenous stimuli, as required.
Fully validated
Liver-on-a-chip models
| Liver model | Cell type | Applications | Required products |
|---|---|---|---|
| Mono-culture | Primary human hepatocytes | HBV, Drug metabolism | PhysioMimix OOC, Liver plates, 3D validated cells |
| Duo-culture | Primary human hepatocytes + Kupffer Cells | HBV, DILI, Immune-mediated toxicity | PhysioMimix OOC, Liver plates, 3D validated cells |
| Tri-culture | Primary human hepatocytes + Kupffer Cells + Stellate Cells | NASH | PhysioMimix OOC, NASH-in-a-box |
Liver-on-a-chip applications
Disease modeling
Safety toxicology
