cnb791 ht logo promo |

Introducing the new PhysioMimix Single-Organ Higher Throughput System

Learn more
CN Bio logo CN Bio logo CN Bio logo CN Bio logo
  • Products
    • Explore our solutions


      PhysioMimix® products enable you to recreate complex human biology and accurately predict human drug responses

      PhysioMimix OOC

      PhysioMimix OOC systems
      Learn more

      Consumables

      • Multi-chip plates
      • 3D validated cells
      • NASH-in-a-box
      Learn more

      Models

      • Single-organ models
      • Multi-organ models

      Support packages

      • PhysioMimix® support packages
  • Applications
    • Discover the applications


      Investigate the application areas that our PhysioMimix® products and services support

      Learn more

      Disease modeling

      • Non-Alcoholic Steatohepatitis
      • Hepatitis B
      • Oncology
      • COVID-19
      Learn more

      Safety toxicology

      • Drug-induced liver injury
      • Immune-mediated toxicities
      Learn more

      ADME

      • Drug absorption
      • Drug metabolism
      • Drug bioavailability
      Learn more
  • Services
    • Studies as a service


      Our team will work collaboratively with you to design a study around your research goals and generate actionable data within weeks

      Learn more
      NAFLD / NASH icons

      NAFLD / NASH

      Drug-induced liver injury icon

      Drug-induced liver injury

      icon adme |

      ADME

      Oncology icon

      Oncology

  • Resources
  • Company
    • About us


      Meet the team, explore our culture and discover what to expect when working with us

      CN Bio team photo
      About us

      Latest news

      • Immune-mediated DILI – Predicting the unpredictable!

      • CN Bio appoints Deepak Singh as Vice President of Sales and Marketing

      • CN Bio extends microphysiological system portfolio with PhysioMimix Single-Organ Higher Throughput System  

      View all news

      Upcoming Events

      • SLAS Europe 2023

      • SOT 2023

      • WORD 2023

      • MPS World Summit 2023

      View all events

      Join the team!

      • Doctoral Researcher (Horizon Marie Curie Doctoral Network Project)

      View all jobs
  • Contact
  • Products
    • PhysioMimix® OOC Microphysiological Systems
    • Consumables
      • PhysioMimix® multi-chip plates
      • 3D validated cells
      • NASH-in-a-box
    • Organ-on-a-chip models
    • Support packages
  • Applications
    • Disease modeling
    • Safety toxicology
    • ADME
  • Services
    • Non-Alcoholic Steatohepatitis
    • Drug-Induced Liver Injury
    • ADME
  • Resources
  • Company
    • About us
    • Events
    • News
    • Careers
  • Contact
  • Skip to main content
  • Skip to footer
Chat live with an expert

Immune-mediated toxicities

How can you predict altered drug responses due to underlying disease?


Inflammation in the liver can arise following a variety of insults. Inflammation can change liver biology and frequently makes hepatocytes more sensitive to insults from hepatotoxicants.

Immune-mediated toxicity is challenging to predict as the root cause is not related to a drug’s mechanism of action or structure, and adverse reactions only occur in a subset of patients.

Most models do not have the complexity to allow the co-culture of liver cells with immune cells. Nor do they allow long enough cultures to explore chronic inflammation effects or disease states on immune-mediated toxicity.

Our solution

By incorporating immune cells into PhysioMimix® organ-on-a-chip (OOC) models, detailed mechanistic studies of the condition in the presence of various inflammatory cues and disease can now be performed to flag susceptibility risks.

Although not exclusive to the liver, this is where adverse reactions are most prevalent. Using the PhysioMimix Immune-mediated DILI Assay, co-cultures of human hepatocytes and immune cells are exposed to the drug of interest in the presence or absence of immuno-stimulation. The model can be used to identify adverse effects on hepatic health and function or changes to the inflammatory profile.

The effects of single acute dose responses or chronic treatment regimens over multiple weeks can be assessed and compared to better predict drugs that induce immune-mediated effects.

Using PhysioMimix disease models, drugs can also be tested in the presence of common liver disorders such as Hepatitis B and NAFLD/NASH to identify patient cohorts with increased DILI susceptibility due to the presence of underlying inflammatory disease.

Immune-mediated toxicity cells

Studying immune-mediated toxicity

Limitations with current techniques

  • Challenging to study immune-tissue level interaction in vitro
  • Poor in vitro to in vivo data translation
  • Unable to recapitulate inflammation driven by disease
  • Challenging to run acute and chronic exposure assays due to shortlived cultures

Advancements with PhysioMimix OOC

  • Immune cells can be incorporated into and circulate around liver microtissues
  • Measurement of clinical biomarkers (e.g. ALT/AST) allows for direct comparison to in vivo data
  • Inflammation can be induced through disease state e.g. NAFLD/NASH
  • Long-term stable tissues enable repeat compound dosing

Flagging enhanced DILI risk due to inflammation


The effect of inflammation on DILI sensitivity can be assessed by testing therapeutics in the PhysioMimix Inflammatory-mediated DILI Assay with or without specific immunomodulators (e.g., LPS).

Rubiano et al., 2020

Kupffer cells bar graph

Immune-mediated toxicity


Following exposure to pro-inflammatory stimuli, liver microtissues produce a range of physiologically relevant cytokines, chemokines, and growth factors that can be altered using different stimuli or inducing disease states.

Sarkar et al., 2017

Immune-mediated toxicity graph

Access our DILI Service


Get instant access to the immune-mediated DILI Assay via our CRO Service. Through a collaborative approach, our experts work with you to plan and execute your study.

Bespoke projects are carried out by our dedicated team of scientists in our CRO facility providing you with actionable data within weeks.

Learn more

Add PhysioMimix OOC into your lab


Harness the power of PhysioMimix OOC in your own lab with the purchase of a single- or multi-organ microphysiological system.

With a growing community of users and support from our experts, there has never been a better time to transition into 3D cell culture.

Learn more

Featured resources

Scientific publications

Biotransformation PK Graphic |

Integrated assessment of diclofenac biotransformation, pharmacokinetics and omics-based toxicity in a 3D human liver-immunocompetent co-culture system

Blogs

blog lung in crisis |

Immune-mediated DILI – Predicting the unpredictable!

Application notes

MPS Fatty Liver graphic |

Microphysiological system for studying fatty liver disease and its impact on drug-induced liver injury

View all Immune-mediated toxicity related resources

Speak to our experts

Speak directly with one of our OOC experts to see how our products and services can support your studies

Request a meeting

Footer

CN Bio logo

332 Cambridge Science Park, Milton Road
Cambridge, CB4 0WN

UK: +44 (0) 1223 737 941

US: +1 415 523 4005

Privacy | Cookies | Terms | Regulatory | Accessibility

©2023 CN Bio Innovations Ltd
Registered No. ‍06517359. VAT No. 978184563

Latest news

  • Immune-mediated DILI – Predicting the unpredictable! March 16, 2023
  • CN Bio appoints Deepak Singh as Vice President of Sales and Marketing March 14, 2023
  • CN Bio extends microphysiological system portfolio with PhysioMimix Single-Organ Higher Throughput System   February 27, 2023

Upcoming events

MPS World Summit 2023 June 26-30, 2023

SLAS Europe 2023 May 22-26, 2023

SOT 2023 March 19-23, 2023

WORD 2023 March 22, 2022