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Explore our solutions


PhysioMimix® is a suite of hardware, consumables and assay protocols that enable you to recreate complex human biology and accurately predict human drug responses.

PhysioMimix OOC

physiomimix-single-and-multi-organ-on-a-chip-systems
Learn more

Consumables

Multi-chip plates
3D validated cells
NASH-in-a-box
Bioavailability assay kit: Human 18
DILI assay kit: Human 24
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Models

Single-organ models
- Liver-on-a-chip model
- Lung-on-a-chip model
Multi-organ models
- Gut/Liver-on-a-chip models

Support packages

PhysioMimix® support packages

Discover the applications


Investigate the application areas that our PhysioMimix® products and services support

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Disease modeling

Metabolic dysfunction-associated steatohepatitis
Hepatitis B
Pulmonary infection
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Safety toxicology

Drug-induced liver injury
Immune-mediated liver injury
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ADME

Drug absorption
Drug metabolism
Drug bioavailability
Oligonucleotide delivery
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Studies as a service


Our team will work collaboratively with you to design a study around your research goals and generate actionable data within weeks

Learn more
icon-nash-1-150x150.png MASLD/MASH
icon-dili-tox-150x150.png Drug-induced liver injury
icon-adme-150x150.png ADME

Speak to an expert about our Bioavailability Assay Kit: Human 18

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PhysioMimix® consumables

Bioavailability assay kit: Human 18


Fast-track your route to a modernized workflow with the only in vitro bioavailability assessment tool on the market

cnb1262 kit mock v1 | Bioavailability Assay Kit

The PhysioMimix Bioavailability assay kit: Human 18 contains everything you need to recreate our human in vitro Gut/Liver-on-a-chip model and Bioavailability assay in your laboratory.

The Bioavailability assay kit: Human 18 bridges the gap between in vitro and in vivo studies to reduce the risk calculus by enhancing your ability to profile human oral bioavailability ahead of in vivo animal and first-in-human trials.

Watch this short video to discover how you can leverage the expertise of two microphysiological system (MPS) providers to recreate our fluidically connected primary human RepliGut®-Planar Jejunum/ PhysioMimix Liver-on-a-chip model using the PhysioMimix Multi-organ System.

Our Bioavailability assay kit: Human 18 supports:

cnb icons stopwatch | Bioavailability Assay Kit

The rapid adoption of Organ-on-a-chip technology by scientists of all experience levels

icon cell | Bioavailability Assay Kit

Instant access to validated tissue models and protocols, developed and refined through years of industry collaboration

icon data | Bioavailability Assay Kit

Prediction of human-relevant ADME parameters by combining data generated from the assay kit with in silico modeling

icon tablets | Bioavailability Assay Kit

Ability to simulate oral and IV drug dosing in a single experiment

Features of our Bioavailability assay kit: Human 18

Use the assay kit to avoid unexpected results in the clinic by removing the limitations of traditional approaches that lead to incorrect estimations of ADME parameters.

  • Modernize your workflow: Eliminate the need for model/assay development and validation with our all-in-one kit
  • Assay kit output: gut and liver ADME estimations, plus oral bioavailability profiling via a fluidically linked multi-organ system
  • Enhanced study design: Use the 18-replicate kit to explore and adapt parameters before in vivo animal studies, reducing the number of animals required.
  • Unique insights: Complement in vivo studies with human in vitro data to flag potential interspecies differences and reduce reliance on animal models.
  • Validated primary human cells: Recapitulate the microarchitecture of the liver and human intestine.
  • Proprietary media: Co-culture validated primary cells (for individual and combined Gut/Liver culture) up to 72 hours.
  • Key metabolic enzyme expression: In both gut and liver, including CYP3A, CYP2C.

* You may require a license to store human cells in your geographic location.


Cell partners

AltisLogo | Bioavailability Assay Kit
LifeSciences 2022 | Bioavailability Assay Kit

RepliGut®-Planar Jejunum sourced from Altis Biosystems and primary human hepatocytes from LifeNetHealth LifeSciences


What’s in the Bioavailability assay kit: Human 18?

Media

MediaVolume
Hepatocyte thawing media50 mL
Basal media (Liver A)110 mL
Basal media (Liver B)110 mL
Basal media (Gut/Liver)190 mL

PhysioMimix plates

Multi-chip platesNumber of plates/replicates
Dual-organ plate (MPS-TL 6)3 plates for 18 replicates  

Supplements

SupplementsVolume
Supplement I1 x 15 μL
Supplement II2 x 5 µL  
Supplement III1 x 25 µL  
Supplement IV1 x 80-100mg (powder)  
Supplement V1 x 12 µL  

Primary human cells

Primary human cells*Number of cells  
Primary human hepatocytes1 vial
RepliGut®- Planar Jejunum kit1 x kit

*Contact us if you require specific donor characteristics or pooled donors

How does our PhysioMimix Bioavailability assay kit: Human 18 compare?

A wide range of preclinical research and development tools is available for scientists. How does the Bioavailability assay kit: Human 18 compare to traditional approaches?

Liver microsome / PHH suspensionGut-Caco-2 Animal PhysioMimix Bioavailability
Human
Human-relevant CYP activity
Intestinal absorption &
hepatic clearance in one
Throughput
High content
Cost$$$$$$$

Explore our Gut/Liver-on-a-chip models


Interconnect and maintain the high functionality of RepliGut®-Planar Jejunum and Liver-on-a-chip models to recapitulate human gut barrier integrity and metabolic capacity in vitro.

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PhysioMimix System in your laboratory


Harness the power of PhysioMimix OOC in your lab with the purchase of a multi-organ microphysiological system.

With a growing community of users and support from our experts, there has never been a better time to transition into 3D cell culture.

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Explore our Bioavailability assay


Find out more about how our groundbreaking assay enables you to uniquely profile human oral bioavailability in vitro.

Better inform lead candidate selection and refine in vivo study design in your lab with more accurate estimations.

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Access via our ADME Service


CN Bio’s dedicated Contract Research Service Team is here to support you. Get instant access to our in-house expertise to better inform your in vivo study design and lead candidate selection.

Through collaboration, our scientists plan and execute standard, or bespoke studies, providing actionable data in weeks.

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Featured resources

News & Blogs

blog lung in crisis | Bioavailability Assay Kit

How in vitro human Gut/Liver models increase confidence in ADME estimations before human trials 

Application notes

cnb1050 connecting the gut and liver v1 | Bioavailability Assay Kit

Connecting the gut and liver

News & Blogs

blog lung in crisis | Bioavailability Assay Kit

6 Challenges in ADME Drug Development

News & Blogs

blog lung in crisis | Bioavailability Assay Kit

Evaluating Caco-2 cell’s gold-standard status in absorption, permeability and bioavailability Studies: Are Their Limitations Justified?

Webinars

cnb1261 yass bioavailability webinar v1 | Bioavailability Assay Kit

A Gut/Liver microphysiological system for profiling human oral bioavailability

Brochures & Flyers

CNB1430 bioavailability assay kit resource v1 | Bioavailability Assay Kit

PhysioMimix Bioavailability assay kit: Human 18 brochure


Frequently asked questions

How do I order a Bioavailability assay kit: Human 18?

Please enquire here and fill out the short form. A member of our expert team will contact you directly.

Enquire

Can the Bioavailability assay kit: Human 18 kit be used for other applications? 

There is always the possibility of additional utility for the kit:

  1. It can be leveraged to study the gut/liver axis
  2. Drug-drug interaction (DDI)*
  3. Toxicology and toxicodynamics*

*not yet available as a validated assay from CN Bio

What are the three media supplied in the Bioavailability assay kit: Human 18 used for? 

Liver A and B Basal media are used for seeding and maintaining primary human hepatocytes in the Multi-chip Dual-organ plate. The Gut/Liver Basal media has been designed for primary Gut/Liver coculture for up to 72 hours to accomadate a drug-dosing window.

What is the shelf life of the Bioavailability assay kit: Human 18 and how do I store it? 

The Bioavailability assay kit: Human 18 will arrive in three drop shipments.

1. CN Bio shipment contains Dual-organ plates (store at room temperature), Liver and Gut/Liver coculture basal media and supplements (store at +4°C, or -20°C as per kit instructions).

2. LifeNet Heatlth shipment contains primary human hepatocytes and should be stored in liquid nitrogen.

3. RepliGut® Planar Jejunum kit will be drop shipped last as this kit needs to be used within within a week of being received by the customer. Cells from Altis Biosystems to be stored in liquid nitrogen with the remaining kit components stored at +4°C.

cnb1015 drug dosing timeline v2 | Bioavailability Assay Kit

Where can I find the Bioavailability assay kit: Human 18 protocol? 

The protocol is supplied with the shipment of your PhysioMimix Bioavailability assay kit: Human 18 along with media preparation instructions, safety data sheets and other documents. 

How are the cells within the Bioavailability assay kit: Human 18 validated for use? 

Primary human hepatocyte donors are first validated for 3D tissue growth, health and metabolic activity following CN Bio’s standard validation process. Adequately performing human hepatocyte donors are then co-cultured with RepliGut®– Planar Jejunum*, following the Bioavailability assay kit: Human 18 SOP, to check that dual-organ (Gut/Liver) functionality is maintained.

*The RepliGut – Planar Jejunum kit is sourced from/ validated by Altis Biosystems.

How can you correlate data generated using the Bioavailability assay kit: Human 18 to the clinic? 

Data generated using the Bioavailability assay kit: Human 18 can be used to predict human-relevant ADME parameters via your in-house in silico modeling.

We plan to commercialize a CN Bio computational modeling tool in the future. Contact us for more information or to enquire about early access.

Is donor information available?

Yes, information is available for all donors and can be requested. Contact us for more information.

Speak to our experts

Request a meeting with one of our OOC experts to see how our products and services can support your studies

Request a meeting

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Latest news

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