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How to Perform Endpoint Media Recovery for Analysis from Organ-on-a-Chip
Not Your Average Joe – Episode 6
Filed under: General OOC
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Think collecting samples from an Organ-on-a-Chip study is complicated?
Many researchers focus on the culture phase of an experiment, but the final step is the most important: endpoint media recovery to generate meaningful biological insight.
In this episode, Isavella from CN Bio’s R&D toxicology team guides Joe, our Director of Sales (Americas), through the final stage of an Organ-on-a-Chip study, demonstrating how to recover endpoint media for downstream analysis using PhysioMimix® Core.
After four weeks of culture, the focus shifts from maintaining tissues and dosing compounds to collecting samples that support metabolite and biomarker detection for further analysis.
In This Video You’ll Learn
This practical demonstration walks through the final stage of an Organ-on-a-Chip experiment and shows how endpoint samples can be prepared for downstream analysis.
- How to recover media from your Multichip plate
Learn the correct approach for collecting media for analysis at the end of your experiment. - Best practices for sampling without disrupting tissues
See how to collect media samples while preserving tissue integrity. - How to store media samples for delayed analysis
Learn how to store collected samples for later downstream workflows. - How to maximise data output at the end of your experiment
Explore practical considerations that maximise the value of each study.
Turning Media Samples into Insight
Generating meaningful data doesn’t end when your experiment concludes.
Once tissues have been cultured and dosed, media samples provide an opportunity to investigate biological responses through biomarker analysis and other downstream workflows.
This episode demonstrates how straightforward media recovery can be, helping researchers prepare high-quality samples ready for additional analysis.
Why Sample Recovery Matters
The ability to recover quality endpoint samples is an important part of generating meaningful experimental data.
Careful sample collection can support:
- Biomarker analysis
- Functional assessment
- Toxicity investigations
- Mechanistic studies
- Longitudinal research programmes
By following a structured workflow, researchers can ensure samples are ready for immediate analysis or future testing.
This content is part of a wider series:
Follow Joe’s journey as he discovers why Organ-on-a-Chip technology is easier to adopt than many researchers expect.
Watch other episodes
Series 1, Episode 1
Getting ready for Organ-on-a-chip – It’s easier than you think!
Series 1, Episode 2
Your First Day with the PhysioMimix® Core. What’s Included? Let’s unbox
Ready to Generate Meaningful Data from Your Organ-on-a-Chip Studies?
Discover how PhysioMimix® helps researchers culture human tissues, generate samples for downstream analysis and investigate complex biological questions using Organ-on-a-Chip technology..
