Organ-on-a-Chip Resources

“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Ortega-Prieto et al., 2019

Despite the exceptional infectivity of the hepatitis B virus (HBV) in vivo, where only three viral genomes can result in a chronicity of experimentally infected chimpanzees, most in vitro models require several hundreds to thousands of viral genomes per cell in order to initiate a transient infection.

3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

Ortega-Prieto et al., 2018

With more than 240 million people infected, hepatitis B virus (HBV) is a major health concern. The inability to mimic the complexity of the liver using cell lines and regular primary human hepatocyte (PHH) cultures pose significant limitations for studying host/pathogen interactions.

Identification And Evaluation Of Patient Serum Isolates As A Source Of Inoculum For 3D in Vitro Cell Culture: A Tool For Drug Discovery

Large et al

Hepatitis B Virus (HBV) is currently a leading healthcare problem affecting more than 240 million people worldwide, despite the availability of a highly effective vaccine. Drug therapies are available on the market for the treatment of HBV, but these rarely provide a cure.

Here, we demonstrate the potential of an in vitro 3D liver MPS to study HBV infection and treatment.

Three-dimensional perfused cell culture enhances the infectability of primary human hepatocytes with Hepatitis B virus

Kostrzewski et al

Research into the biology and treatment of hepatitis B virus (HBV) requires an in vitro infection model that fully supports all the steps of the HBV life cycle and accurately recapitulates virus-host interactions. However, the molecular mechanisms underlying HBV infection remain poorly understood. Here, we show the potential of a perfused 3D cell culture system over standard 2D cell culture to investigate the infectivity of PHH by HBV.