PhysioMimix® consumables
DILI assay kit: Human 24
Predict more complex, latent and immune-related hepatotoxicity with our in vitro DILI assay kit: Human 24

The PhysioMimix DILI assay kit contains everything you need to recreate our FDA-recognized assay in your own laboratory.
The DILI assay kit: Human 24 enables you to be better informed ahead of in vivo animal and first-in-human studies by going beyond intrinsic DILI predictions. Identify more complex, latent and immune-related events with this all-in-one kit and our PhysioMimix OOC range of single- and multi-organ microphysiological systems (MPS).
Watch this short video to discover how to ensure only the most promising therapeutic candidates progress by unlocking deeper mechanistic insights.
Our DILI assay kit offers:
Effortless adoption – Easily and rapidly adopt the latest Organ-on-a-chip technology
All-in-one kit – Instant access to high-quality validated cells and reagents to reliably reproduce our assay in your workflow
Proven solution – Benefit from decades of expertise refined through years of industry collaboration
Human relevance – Provides a viable solution for new drug modalities with human-specific targets and pathways
Comprehensive support – Instrument and application support for all elements of your assay kit
Features of our DILI assay kit
Bridge the knowledge gap between traditional in vitro and first-in-human studies with our DILI assay kit: Human 24.
- 24-replicate kit: profile 8 conditions in triplicate, including positive and negative controls
- Complement in vivo studies: human in vitro data flags potential interspecies differences and reduces reliance on animal model data
- Unique Insights: from 3D liver tissues, comprised of primary human hepatocytes and Kupffer cells, over 14 days of culture
- Key metabolic enzyme expression: including CYP enzymes
- Mechanistic profiling: large recoverable volumes of tissue and media for DILI pathway profiling
- Enhanced clinical translatability: key biomarkers including ALT/AST, albumin, urea, LDH
Speak to an expert about our DILI Assay Kit: Human 24
What’s in the DILI assay kit: Human 24?
Media
Media | Volume |
Liver Basal A Media | 150 mL |
Liver Basal B Media | 500 mL |
Thawing Media | 50 mL |
PhysioMimix plates
Multi-chip plates | Number of plates/replicates |
Liver-12+ plates | 2 plates with 24 replicates |
Supplements
Supplements | Volume |
Supplement I | 7 x 10 µL |
Positive Control | 4 x 50 µL |
Negative Control | 4 x 50 µL |
Primary human cells
Primary human cells | Number of cells |
Primary Human Hepatocytes | 2 vials |
Kupffer cells | 2 vials |
Validation report
Our sample report describes the extensive process of validating the primary human hepatocytes and Kupffer cells used in the DILI assay kit: Human 24.
The quality control procedures ensure the formation of stable, human-relevant liver microtissues that are metabolically active and immune-competent.
How does our DILI assay kit compare?
A wide range of preclinical research and development tools is available for scientists. How does the DILI assay kit: Human 24 compare to traditional approaches?
Liver monolayer / spheroid | Animal model | PhysioMimix DILI assay | |
Human | |||
Human-relevant CYP activity | |||
Mechanistic toxicity | |||
Advanced drug modalities | |||
Throughput | |||
Cost | $ | $$$ | $$ |
Explore our Liver-on-a-chip models
Recreate the 3D multi-cellular architecture of the liver using perfused scaffolds. Achieve longer-term viability, enhanced functionality & high metabolic activity.
PhysioMimix OOC in your lab
Harness the power of PhysioMimix OOC in your lab by purchasing a single- or multi-organ microphysiological system. There has never been a better time to transition into OOC, so what are you waiting for?
Explore our DILI assay
Deeper mechanistic insights from our predictive human DILI assay enable you to refine preclinical design by narrowing down candidate selection and be better prepared for the clinic when developing advanced drug modalities.
DILI contract research services (CRS)
Get instant access to the PhysioMimix DILI assay via our CRS. Our dedicated team of CRS scientists work collaborately with you to deliver actionable data within weeks for standard and bespoke projects.
Frequently asked questions
How do I order a DILI assay kit: Human 24?
Please enquire here and fill out the short form. A member of our expert team will contact you directly.
Why are there two types of media supplied with the DILI assay kit?
The DILI assay kit: Human 24 is supplied with two Liver Basal Media (Basal A and B). Each needs to be combined with a supplement and bovine serum to support the generation of liver tissue:
- Liver Seeding Media: made from Liver Basal A Media plus supplements, is required for cell seeding in Liver-12+ plates and the first 24 hours of culture.
- Liver Maintenance Media: made from Liver Basal B Media plus supplements, is used for the remainder of the experiment, including drug dosing.
Sufficient Liver Basal B Media is provided to run an experiment using two Liver-12+ plates, for a 14-day cell culture period.
What is the shelf life of the DILI assay kit?
Individual elements of the kit have a different shelf life, and thus it is important to keep track of these when using the kit.
Component | Manufacturer | Shelf life | Storage conditions |
Liver media and supplements | CN Bio | Up to three months | Supplements at -20°C Liver media at +4⁰C |
Liver-12+ plate | CN Bio | Up to 12 months | Dry and temperature-controlled environment (no higher than +25⁰C) |
Where can I find the DILI assay kit protocol books?
The protocol is comprised of three documents:
- List of Materials. This details all materials required to run a 14-day study using the kit (including any materials not supplied as part of the kit). A printed version is provided within the kit and a printable version will be emailed to you.
- Liver Tissue Generation Protocol Book. This document lists all materials required for media preparation and the generation of 3D liver tissues. Plus a step-by-step protocol. This will be supplied by email and is downloadable via a QR code on the Quick Start Guide (included in the kit).
- Drug Dosing Protocol Book. The third document lists all materials required for a 10-day drug dosing experiment and associated media preparation. Plus a step-by-step protocol for drug dosing. This will be supplied by email and is downloadable via a QR code on the Quick Start Guide (included in the kit).
What literature is supplied with my DILI assay kit shipment
- Safety Data Sheets.
- Quick Start Guide.
- List of materials required to run the assay.
How are the primary human cells in the DILI assay kit validated?
Firstly, primary human hepatocytes (PHH) are validated in isolation, following our standard validation process.
Once PHH donors have been validated, they are co-cultured with Kupffer cell donors, following the kit’s protocol, to ensure that the functionality of hepatocyte and Kupffer cells is maintained. In addition, validated co-cultures are tested for DILI response using the positive control included in the kit.
Once a donor combination has been validated, it is reserved for kit use.
Is donor info available for the primary human cells used in the DILI assay kit?
Yes, information is available for all donors upon request. Contact us for more information.
How many DILI assay kit boxes should I expect to receive, and how many components are within each box?
Customers will receive a minimum of five boxes per kit order. The kit will arrive in two shipments: CN Bio (three boxes) and LifeNet Health (two boxes) and contains storage condition information for the individual elements of the kit.
What is the standard experimental timeline for the DILI assay kit?
A standard experiment using the PhysioMimix DILI Assay kit: H24 takes up to two weeks and is usually split between two parts:
- Tissue generation: primary liver tissue takes four days to stabilize. Quality control testing is carried out on day 4.
- Drug dosing: occurs over the next ten days, depending on the toxicity of the tested compounds.
In addition to the DILI assay kit, what do I need to start my experiment?
Anyone purchasing the kit must have a PhysioMimix OOC System with two Single-organ drivers (MD6).
You will also need some common cell culture reagents including FBS (bovine serum) and Bovine serum albumin (BSA) to prepare the Liver Seeding and Maintenance Media. aPES filters are also required to filter media stock solutions during media preparation. We also strongly recommend using wide-bore 200 µL pipette tips for seeding primary liver cells to reduce damage and stress.
Standard cell culture equipment, such as pipettes, pipette tips, 15- and 50-mL Falcon tubes, PBS, Trypan blue and haemocytometer, is also required.
We recommend purchasing Glo-P450 test kits (Promega) to QC liver tissue for metabolic activity, plus testing for LDH and Albumin production to ensure that liver tissues are healthy and functional.
How many DILI assay kits can I use at the same time?
The PhysioMimix Single-organ System can run up to six plates simultaneously. Each kit contains two Multi-chip Liver-12 + plates, therefore you can use one, two or three kits simultaneously. To run at full capacity (72 wells /run), you will need one Controller, two docking stations and six Single-organ drivers. Customers with only one docking station and three drivers can only use one kit/run.