In vitro ADME services

A unique opportunity to measure drug ADME in vitro without using animals

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Use our in vitro ADME services to investigate drug metabolism, metabolite identification, permeability, and bioavailability. Studies are conducted using human highly functional, metabolically competent single- and multi-organ models that are highly predictive of clinical results. Combine with computational modeling to translate experimental data into predictions of human ADME behaviour.

Service overview:

We offer a range of single- and multi-organ models to derive human-relevant data to better inform the selection of lead candidates with desirable ADME properties.

Through this service, you can utilize a combination of our expertise and highly metabolically active, long-term cultures to derive unique in vitro data.

• Hepatic clearance including low clearance compounds and metabolite identification, including Phase I and II metabolites
• Gut metabolism and permeability
• Lung permeability in upper and lower airway models
• Bioavailability prediction using mechanistic mathematical modeling of experimental data
• Method developed with protein-free cell culture medium in conjunction with low non-specific binding multi-chip assay plates

Customer feedback

How the service works:

PhysioMimix Core enables the profiling of human ADME parameters using predictive human liver, gut, lung, or multi-organ model combinations.

Our in vitro service generates novel insights into the human body’s effect on drugs, previously only possible using animal models.

Your dedicated contact will work collaboratively with you from the start to the end of the project.

1. Design and finalize the experimental plan, optionally incorporating computational modeling to support and refine the design
2. Customer supplies required amount of drug(s)
3. Growth and preparation of organ models prior to treatment – between four and 21 days, dependent on assay selected
4. Compound dosing and sample collection up to four days depending on the model.
5. One to two weeks to run endpoint assays, analyze data, and complete the report 
6. ~ two months to complete the study from receiving order 
7. Media samples sent to customer, or third party for LC-MS analysis
8. Optional access to computational modeling expertise to translate bioavailability data into a human prediction

Standard liver drug metabolism cell culture timeline

Standard gut/liver drug bioavailability cell culture timeline

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Endpoint measurements

Included, but are not limited to:

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