Barrier Models

CN Bio’s PhysioMimix™ MPS-T12 and MPS-TL6 consumable plates can be used with Transwell® inserts to perform barrier model studies. This provides a simple and adaptable Microphysiological System (MPS) for modelling single cell barriers through to complex co-culture systems.

The platform allows for specific barriers such as the gut, lung or skin to be generated and cultured in the presence of microfluidic perfusion to recreate a physiologically relevant microenvironment.

CN Bio’s barrier models can be used to study drug absorption, metabolism, toxicity and disease.

Gut-on-a-Chip

CN Bio’s multi-well PhysioMimix™ MPS-T12 consumable plate can be used with Transwell® inserts to model the human intestine. Microfluidic perfusion of the tissue enables a barrier with physiology relevant 3D architecture to be recreated that more accurately replicates in vivo conditions versus standard colon adenocarcinoma (Caco-2) cells in static 2D monoculture. This overcomes the issue of under-predicting paracellular drug transport. This in vitro intestine model can be used to study drug absorption, inflammation and barrier integrity.

Gut tissue Morphology

Barrier integrity (TEER)

Gut permeability

Lung-on-a-chip

CN Bio’s multi-well PhysioMimix MPS-T12 consumable plate, can be used with Transwell® inserts to create a Lung-on-a-chip system whereby lung epithelial cells and endothelial cells are grown in a liquid-liquid or air-liquid interface. This model enables the absorption and permeability of inhaled compounds to be tested.

Additionally, Lung-on-a-chip technology can be used to model disease states, including SARS-CoV-2 infection (COVID-19). Our 3D model in development will feature an organotypic air-liquid interface, which has more utility than the current organoid approach where cilia within the structure make it difficult to study viral infection.

The PhysioMimix MPS-T12 consumable plate plates are designed to provide optimal perfused cell culture conditions for:

  • Primary cells
  • Stem cell-derived cells
  • Organoids or Spheroids
  • Commercial inserts (such as MucilAir® or EpiSkin©)
  • Immortalized cell lines

 

Multi-Organ Barrier Models

CN Bio’s PhysioMimix Multi-Organ Microphysiological System and Transwell-Liver MPS-TL6 consumable plates enable our best-in-class Liver-on-a-chip model to be interconnected to a Barrier Model such as gut, or lung to study reactive metabolite driven toxicity, whilst simultaneously evaluating drug absorption and metabolism. In addition, CN Bio is collaborating with world-leading researchers in the development and use of single and multi-organ COVID-19 models to investigate virology and the systemic immunologicaly effects of the infection.

PhysioMimix MPS-TL6 consumable plates are designed to provide optimal perfused cell culture conditions for:

  • Primary cells
  • Stem cell-derived cells
  • Precision cut tissue slices
  • Organoids, spheroids
  • Commercial inserts (such as MucilAir® or EpiSkin©)
  • Immortalized cell lines

 

Related content

SCIENTIFIC PAPER

Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies

Download paper

SCIENTIFIC PAPER

Integrated gut/liver microphysiological systems elucidates inflammatory inter‐tissue crosstalk

Download paper

APPLICATION NOTE

Drug metabolismin a gut-liver microphysiological system

Download Application note

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