Validation of a high-throughput MPS for 3D culturing of primary hepatocytes

A high-throughput 3D MPS model supported the long-term culture of primary human hepatocytes under perfused conditions. Across two donors, the Liver-48 format maintained hepatocyte viability and consistent liver-specific function over 14 days, including robust albumin production. Minimal differences in lactate dehydrogenase release and uniform microtissue formation were observed compared with the established Liver-12 format

Engineering and biological validation demonstrated consistent flow across the Liver-48 plate and showed that scaling experimental capacity four-fold did not compromise liver-specific function or physiological relevance. Together, these findings support the use of high-throughput 3D MPS models to generate robust, reproducible, and human-relevant data at greater scale for drug discovery and development.