Formulating a New Approach to Pharmacokinetics and Cell State Control

Watch this webinar to learn:

• Understand the biological implications of sequential batch feeding of cells in conventional in vitro experiments.
• Appreciate how microfluidic systems can create a different time-dependent formulation of multiple drugs or other compounds to each well of a multi-well plate.
• Recognize the opportunity that continuous, controlled media exchange offers for the culture and study of adherent and suspension cells, organoids, and organs-on-chips.

Most in vitro, well-plate biological experiments require a media change every day or two, with cyclic variations in nutrient and metabolite concentrations leading to physiologically unrealistic gene expression and metabolism. Typical step-changes in drug concentrations do not reflect in vivo pharmacokinetic (PK) exposure profiles, which can lead to inaccurate ranking of oncological drug effectiveness.

State-of-the-art microfluidic pumps and valves enable the creation of Multi-Well Microformulators that impose on each well a different physiological PK profile, circadian rhythm, or temporal changes of growth factors and signalling molecules. This technology enables dynamic exposure response studies, optimization of stem-cell differentiation, and thousand-channel microchemostats.

View our Q&A document from the live event.

Speaker Information:

Professor John Wikswo

Professor of Biomedical Engineering, Molecular Physiology
& Biophysics, and Physics, Vanderbilt Univeristy