In the realm of pharmaceutical research, Caco-2 cells have long been heralded as the gold standard for studying intestinal absorption and permeability. These human epithelial colorectal adenocarcinoma cells are prized for their ability to differentiate into enterocyte-like cells, forming tight junctions that mimic the intestinal barrier. However, as with any model system, Caco-2 cells come … Continue reading Evaluating Caco-2 cell’s gold-standard status in absorption, permeability and bioavailability studies: Are their limitations justified?